Barnsley haven't made it past the third round in the EFL Cup since 2009 and they will have to upset the odds against Premier League Brighton to halt that sorry sequence.
The League One side have home advantage as they bid to spring a shock against their top-flight opponents.
Their form has been pretty decent this term, with six wins from 10 games, but they head into this cup tie on a downer after losing 1-0 at Blackpool on Saturday.
Brighton also had a disappointing weekend as they threw away a 2-0 lead to draw at home against Spurs, with a goals and card bet landing for Slips Tips after he backed an open game.
The Seagulls have enjoyed a few good FA Cup runs in recent years, reaching the semis in 2019 and 2023, but haven't had the same success in the EFL Cup.
Brighton last got beyond the fourth round in 1979 so they will be hoping a win in Barnsley will set the stage for some EFL Cup heroics this season.
How the bookies view it: No shock to Brighton's system
BoyleSports don't fancy Barnsley's chances as they make the Tykes 11/1 outsiders for a win in 90 minutes. Brighton are just 2/7 and the draw pays 9/2.
Recent head to head: Seagulls seeking a hat-trick
These sides last faced off in the Championship back in season 2016/17 and Brighton won 2-0 in both games. But Barnsley just edge the overall head to head, with 17 wins, 16 defeats and 10 draws.
Players to watch: Stefanos set to shine?
Greek kid Stefanos Tzimas was let off the leash in the last round by Brighton and scored twice in their 6-0 rout of Oxford. He could get another outing in this tie and is 11/10 for anytime scorer at Bet365.
Davis Keillor-Dunn hit the 20-goal mark for Barnsley last term and is already on five for the new campaign. He looks their best bet for a goal here at 7/2 with Bet365.
Probable line-ups
Seagulls boss Fabian Hurzeler made lots of changes for their last EFL Cup tie against Oxford so expect plenty of stars to get a breather. Barnsley should be close to full strength as they bid to pull of a major shock.
Barnsley: Cooper, Shepherd, Ogbeta, Watson, Earl, Vickers, Phillips, McGoldrick, Bland, Connell, Keillor-Dunn.
Brighton and Hove Albion: Steele, Wieffer, Boscagli, Kadioglu, Veltman, Coppola, Gruda, Hinshelwood, Milner, Gomez, Tzimas.
Anything else catch the eye?
Brighton were pretty ruthless away from home in the last round as they smashed six past a team from the Championship.
Barnsley are a tier below Oxford so the Seagulls could get more joy and you can boost their odds to 11/10 by backing a win to nil.
Hurzeler's side killed the game off early against Oxford as they were 2-0 up inside 20 minutes. That makes over 1.5 first half goals worth a punt here at 5/4 with BoyleSports.
Brighton haven't won more than five corners in any of their games this season so there could be some value in betting against under 10 flag kicks in this cup tie at evens with Bet365.
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